Cyclin D2 expression in chronic myelogenous leukemia.
- Author:
Jun-min SONG
1
;
Dong XU
;
Er-jin FAN
;
Shi-rong XU
;
Dong LI
;
Chun-hua ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Cyclin D2; Cyclins; analysis; genetics; Flow Cytometry; Genes, abl; Humans; K562 Cells; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; metabolism; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hematology 2004;25(2):103-105
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between cyclin D2 and P210(BCR/ABL) tyrosine kinase in chronic myelogenous leukemia (CML).
METHODSRT-PCR, Western blot and flow cytometry were performed to detect the expression of cyclin D2 in K562 cells and in K562-ib-eGFP cells which express intracellular single-chain antibody (sFv, intrabody) against ABL tyrosine kinase domain.
RESULTSCyclin D2 expression in K562-ib-eGFP cells was 18.90% which was lower than that of control K562 cells (48.10%), and the number of S-phase cells in K562-ib-eGFP was 40.40% which was much lower than that in K562 cells (64.34%).
CONCLUSIONCyclin D2 is a potential down-stream signal molecule of the p210(BCR/ABL) tyrosine kinase in CML. The altered expression of cyclin D2 may contribute to the over proliferation of CML cells.
