Study of antileukemic effect of CpG-oligodeoxynucleotides treated cord blood.
- Author:
Chun-yan CHEN
1
;
Ji-hui JIA
;
Xiang-lin PAN
;
Ya-bin ZHOU
;
Wei-ming ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adjuvants, Immunologic; pharmacology; Antigens, CD; blood; Cytotoxicity, Immunologic; Dose-Response Relationship, Drug; Fetal Blood; cytology; Humans; K562 Cells; Leukemia; therapy; Lymphocytes; drug effects; immunology; Oligodeoxyribonucleotides; pharmacology; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hematology 2004;25(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the antileukemic effect of lymphocytes from cord blood treated by CpG-oligodeoxynucleotides (CpG-ODN).
METHODSLymphocytes from cord blood were exposed to different oligodeoxynucleotides containing a panel of CpG-ODN and were cultured with K562 cells. The cytotoxic effects were detected by MTT method. Immunological markers of cord blood treated by CpG-ODN(3) which showed highest activity were measured with flow cytometry.
RESULTSDifferent CpG-motifs have different immunostimulatory activity and CpG-ODN(3) has the highest one. After treated by CpG-ODN(3), NK killing activity to K562 cells increased in a dose-dependent manner, and CD(3), CD(4), CD(19) and CD(56) increased to (60.6 +/- 7.9)%, (40.2 +/- 3.5)%, (22.4 +/- 1.9)% and (15.5 +/- 3.1)%, respectively.
CONCLUSIONCpG-ODN could reinforces the immunological competence of cord blood lymphocytes and their effects on K562 cells. This provides a new approach to reinforce the antitumor effects of cord blood.
