HUP98-HOXA9 transgenic mice are susceptible to N-ethyl-N-nitrosourea stimulation in leukemogenesis.
- Author:
Shun-yuan LU
1
;
Ming-min GU
;
Yang WANG
;
Yi TAN
;
Yue-ping SUN
;
Long WANG
;
Hui KONG
;
Zhen-yu LU
;
Zhu-gang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; metabolism; pathology; Disease Models, Animal; Ethylnitrosourea; Female; Gene Expression Regulation, Leukemic; Genotype; Homeodomain Proteins; biosynthesis; genetics; Humans; Leukemia, Myeloid; blood; chemically induced; genetics; Male; Mice; Mice, Transgenic; Nuclear Pore Complex Proteins; biosynthesis; genetics; Oncogene Proteins, Fusion; biosynthesis; genetics; Phenotype; Plasmids; Reverse Transcriptase Polymerase Chain Reaction; Transfection
- From: Chinese Journal of Hematology 2004;25(5):257-261
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to investigate the leukemogenic potential of NUP98-HOXA9 fusion gene in vivo.
METHODSMolecular cloning technology was used to construct NUP98-HOXA9 transgenic plasmid and NUP98-HOXA9 transgenic mice were generated. The genotype and phenotype of the NUP98-HOXA9 transgenic mice were analyzed by PCR, RT-PCR and colony-forming assay. The effect of N-ethyl-N-nitrosourea (ENU) stimulation on the transgenic mice was analyzed by peripheral blood count, bone marrow (BM) cells morphology pathological examination.
RESULTSThe transgenic expression was detected in 5 independent lines of NUP98-HOXA9 transgenic mice, but no expected phenotypes was found in 2 year follow-up. Upon ENU stimulation, 2 of 10 transgenic mice developed myeloid leukemia, suggesting that NUP98-HOXA9 transgenic mice have increased susceptibility to ENU mutagenesis in leukemogenesis.
CONCLUSIONThe fusion gene expressed in BM cells of NUP98-HOXA9 transgenic mice. It seems that the expression of the fusion gene is insufficient to trigger leukemogenesis. However, the increased susceptibility to ENU mutagenesis suggests that NUP98-HOXA9 fusion gene might play a potential role in leukemogenesis.