Development of human myeloid leukemia-like phenotype in NUP98-PMX1 transgenic mice.
- Author:
Yang WANG
1
;
Ming-min GU
;
Yi TAN
;
Shun-yuan LU
;
Long WANG
;
Hui KONG
;
Yue-ping SUN
;
Zhen-yu LU
;
Sai-juan CHEN
;
Zhen-yi WANG
;
Zhu-gang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Cells; metabolism; pathology; Disease Models, Animal; Female; Flow Cytometry; Gene Expression Regulation, Leukemic; Green Fluorescent Proteins; genetics; metabolism; Humans; Leukemia, Myeloid; genetics; pathology; Male; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Mice, Nude; Mice, Transgenic; NIH 3T3 Cells; Nuclear Pore Complex Proteins; genetics; metabolism; Oncogene Proteins, Fusion; genetics; metabolism; Phenotype; Plasmids; Recombinant Fusion Proteins; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transfection
- From: Chinese Journal of Hematology 2004;25(5):262-265
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to investigate the leukemogenic potential of NUP98-PMX1 fusion gene in vivo.
METHODSNUP98-PMX1 transgenic mice were generated, in which the fusion gene was driven by hCG promoter and expressed in myeloid cells at early stage of differentiation. Molecular cloning technology was used to construct NUP98-PMX1 transgenic plasmid. The genotype and phenotype of the NUP98-PMX1 transgenic mice were analyzed by PCR, RT-PCR, peripheral blood count (PBC), bone marrow (BM) cells morphology and pathological examination.
RESULTSNIH3T3 cells transfected with NUP98-PMX1 fusion gene grew faster, formed colonies in soft agar, and developed tumors in 10 inoculated nude mice. Among 8 disordered NUP98-PMX1 transgenic mice, 4 developed myeloid leukemia-like phenotype, including 3 resembling human chronic myeloid leukemia.
CONCLUSIONNUP98-PMX1 has oncogenic activity and plays a crucial role in leukemogenesis.
