Gene expression profile of yolk sac and fetal liver in mouse.
- Author:
Jun ZHOU
1
;
Qing-hua ZHANG
;
Long WANG
;
Jing FANG
;
Hai-hong WANG
;
Sai-juan CHEN
;
Zhu CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antigens, Ly; genetics; metabolism; Cell Differentiation; genetics; Flow Cytometry; Gene Expression Profiling; Gene Expression Regulation, Developmental; Liver; cytology; embryology; metabolism; Membrane Proteins; genetics; metabolism; Mice; Mice, Inbred C57BL; Proto-Oncogene Proteins c-kit; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Time Factors; Yolk Sac; cytology; embryology; metabolism
- From: Chinese Journal of Hematology 2004;25(5):266-268
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo better understand the mechanisms of the fetal hematopoiesis turn over from primitive to definitive hematopoiesis through the expression level of c-kit(+) and sca-1(+), and major characters of gene expression profile of these cells.
METHODSc-kit and sca-1 expression level were monitored with fluorescence activated cell sorting (FACS) of the mononuclear cells from mouse yolk sac and fetal liver, while gene expression profile was carried out with EST sequencing strategy.
RESULTSThe Sca-1(+) cells were increased while the c-kit(+) cells decreased with the embryonic development. Through profiling the functionally identified known genes, most of the highly expressed were globin genes, especially of embryonic types.
CONCLUSIONThe erythropoiesis played a key role in early fetal hematopoiesis in mammalian.
