The initiation of G2/M checkpoint by diallyl disulfide requires the activation of p38 MAP kinase in HL-60 cells.
- Author:
Li-ming TAN
1
;
Meng-xia ZHANG
;
Hong-mei LUO
;
Yong-zhi ZENG
;
Jian-min LI
;
Ze-wen CUI
;
Xiao-hong ZHANG
;
Sheng-song TANG
Author Information
- Publication Type:Journal Article
- MeSH: Allyl Compounds; pharmacology; Blotting, Western; CDC2 Protein Kinase; genetics; metabolism; Cell Division; drug effects; Cell Proliferation; drug effects; Cell Survival; drug effects; Disulfides; pharmacology; Dose-Response Relationship, Drug; Enzyme Activation; drug effects; Flow Cytometry; G2 Phase; drug effects; Gene Expression; drug effects; HL-60 Cells; Humans; Phosphorylation; drug effects; Reverse Transcriptase Polymerase Chain Reaction; cdc25 Phosphatases; genetics; metabolism; p38 Mitogen-Activated Protein Kinases; genetics; metabolism
- From: Chinese Journal of Hematology 2004;25(5):273-276
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the molecular mechanisms of G(2)/M checkpoint initiated by diallyl disulfide (DADS) in HL-60 cells.
METHODSCell viability was determined by MTT assay. Cell cycle was assayed by flow cytometry. The expression of phospho-p38, Cdc25B and Cdc2, and p38 mRNA were measured by Western blotting and RT-PCR, respectively.
RESULTSAfter treatment with DADS at 5 - 160 micro mol/L for 0 - 72 h, the growth of HL-60 cells were suppressed in a concentration-dependent manner and the inhibitory effect of DADS (20 micro mol/L) was similar to that of ATRA (10 nmol/L) (P > 0.05). Incubation of HL-60 cells with DADS (20 micro mol/L) for 12 h could activate G(2)/M checkpoint and increase the expression of phospho-p38 MAPK, followed by the expression of phospho-Cdc25B and phospho-Cdc2 (P < 0.05). SB202190, a specific inhibitor of p38 MAPK, markedly blocked the phosphorylation of p38 MAPK, Cdc25B and Cdc2 (P < 0.05).
CONCLUSIONDADS could induce the G(2)/M arrest in HL-60 cells which may be involved in the activation of p38 MAP kinase.
