Analysis of methylation status of the promoter of mdr1 gene in K562 and K562/DNR cells.
- Author:
Feng GAO
1
;
Jing-dong ZHANG
;
Yun-peng LIU
;
Xiang-lan LU
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; genetics; metabolism; Base Sequence; DNA Methylation; Flow Cytometry; Gene Silencing; Humans; K562 Cells; Promoter Regions, Genetic; genetics; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; methods
- From: Chinese Journal of Hematology 2004;25(5):293-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the methylation patterns of mdr1 gene promotor in both K562 cell and K562/DNR cells and the relationship between promotor methylation and P-gp expression.
METHODSmdr1 gene expression was analyzed by flow cytometry and RT-PCR, methylation status of the promotor of mdr1 gene by bisulfite-sequencing (including two GC-box).
RESULTSmdr1 gene was found methylated at GC-box and not expressed in K562 cells, but unmethylated and expressed respectively in K562/DNR cells.
CONCLUSIONThe methylation patterns of mdr1 gene promotor in K562/DNR and K562 cells were different. mdr1 gene silencing was associated with the promotor methylation.
