Apoptosis and proliferation of CD34 positive bone marrow cells in patients with polycythemia vera.
- Author:
Jie BAI
1
;
Zong-hong SHAO
;
Hong LIU
;
Jun SHI
;
Guang-sheng HE
;
Yan-ran CAO
;
Mei-feng TU
;
Zhen-zhu CUI
;
Hai-rong JIA
;
Juan SUN
;
Lin-sheng QIAN
;
Tian-ying YANG
;
Chong-li YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Annexin A5; analysis; Antigens, CD34; analysis; Apoptosis; Bone Marrow Cells; cytology; Cell Division; Female; Humans; Male; Middle Aged; Polycythemia Vera; pathology
- From: Chinese Journal of Hematology 2004;25(4):195-197
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the apoptosis and proliferation of CD(34) positive (CD(34)(+)) bone marrow cells (BMC) in patients with polycythemia vera (PV).
METHODSThe expression of Annexin V and Ki67 of the CD(34)(+) BMC in 20 PV patients and control cases [10 essential thrombocythemia (ET), 12 normal persons] were assessed by bicolor flow cytometry (FCM), and the correlation between apoptosis and clinical situation was analysed in PV patients.
RESULTSThe Annexin V expressions of CD(34)(+) BMC were (15.96 +/- 1.45)% in PV patients and (15.53 +/- 1.76)% in ET patients which were lower than that in normal subjects [(23.61 +/- 3.89)%, (P < 0.05)]. The Ki67 expression of CD(34)(+) BMC was (48.79 +/- 11.68)% in PV patients and (49.60 +/- 9.98)% in ET patients, which were significantly higher than that in normal controls (33.87 +/- 6.82)%. The ratio of apoptosis/proliferation in PV patients was 0.33 +/- 0.10 and in ET patients 0.32 +/- 0.02 which were significantly lower than that in normal controls 0.72 +/- 0.11 (P < 0.01). The apoptosis of CD(34)(+) BMC was negatively correlated with the hemoglobin (Hb) levels (r = -0.481, P = 0.037), white blood cells (WBC) (r = -0.538, P = 0.026) and the numbers of endogenous erythroid colony (EEC) (r = -0.632, P = 0.50), and the ratio of apoptosis/proliferation was negatively correlated with the Hb (r = -0.537, P = 0.018) and WBC (r = -0.667, P = 0.003) in PV patients.
CONCLUSIONThere were lower apoptosis and higher proliferation in CD(34)(+) BMC of PV patients. Lower apoptosis was correlated with the severity of the disease.
