Effect of curcumin on STAT5 signaling molecule in K562 cells.
- Author:
Wei-Hong CHEN
1
;
Yan CHEN
;
Jun-Xia GU
;
Jing HE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; drug effects; Curcumin; pharmacology; Gene Expression Regulation, Leukemic; drug effects; Humans; K562 Cells; Leukemia; genetics; metabolism; physiopathology; STAT5 Transcription Factor; genetics; metabolism; Signal Transduction; drug effects
- From: Chinese Journal of Hematology 2004;25(3):151-153
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of curcumin on STAT 5 signaling molecule in K562 cells and its molecular mechanism of antileukemia.
METHODSCell proliferation was studied by tetrazolium dye assay. The expressions of STAT5 mRNA and protein were assayed by in situ hybridization, and Western blotting respectively.
RESULTSCurcumin could inhibit K562 cell proliferation in a time- and dose-dependent manner. The percentage of STAT5-positive cells was 19% in curcunin group, significantly less than 31% of that in K562 cell group (P < 0.01). The A value of the expression level of STAT5 protein in curcumin group was 15 266 +/- 769, significantly less than 25 781 +/- 1240 of that in K562 cell group (P < 0.01).
CONCLUSIONThe expressions of STAT5 mRNA and protein in K562 cells were inhibited by curcumin and curcumin could inhibit K562 cell proliferation.
