Effect of ATRA and DNR on the expression and secretion of VEGF in leukemic cells.
- Author:
Chen WANG
1
;
Fang-Yuan CHEN
;
Chun-Hong GU
;
Jie-Ying HAN
;
Hua ZHONG
;
Ji-Hua ZHONG
;
Ye TENG
;
Ren-Rong OUYANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Daunorubicin; pharmacology; Gene Expression Regulation, Leukemic; drug effects; HL-60 Cells; Humans; Leukemia; drug therapy; genetics; metabolism; physiopathology; Tretinoin; pharmacology; Vascular Endothelial Growth Factors; genetics; metabolism
- From: Chinese Journal of Hematology 2004;25(3):171-174
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cells affected by all-trans retinoic acid (ATRA) and daunorubincin (DNR) respectively.
METHODSSemi-quantitative RT-PCR and ELISA were used to study the expression of VEGF mRNA and secretion of VEGF protein in NB4 and HL-60 cell lines treated by ATRA and DNR respectively.
RESULTSVEGF was expressed in both NB4 and HL-60 cells. The expression of VEGF mRNA and secretion of VEGF protein could be down-regulated by ATRA and DNR respectively in a time and dose dependent manner.
CONCLUSIONBesides inducing apoptosis and restraining proliferation of leukemic cells, ATRA and DNR exerted their anti-leukemia effects by reducing angiogenesis via reduction of angiogenic reaction stimulating signals.
