Gene expression profile in early differentiation of K562 cells induced by matrine.
- Author:
Yan ZHANG
1
;
Ling-Di MA
;
Yu-Juan HE
;
Ji-Kai JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacology; Cell Differentiation; drug effects; genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; drug effects; Humans; K562 Cells; cytology; drug effects; metabolism; Oligonucleotide Array Sequence Analysis; Quinolizines; pharmacology
- From: Chinese Journal of Hematology 2004;25(6):342-345
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare gene expression profile in K562 cells induced by matrine, so as to screen for differentiation related genes.
METHODSK562 cells were exposed to 0.1 mg/ml matrine for 3 hours, and gene expression profiles in matrine-treated and non-treated cells were studied by cDNA microarray.
RESULTSFrom 8465 screened genes, 30 differentially expressed genes were found. Among them 18 showed decreased expression including oncogene and proto-oncogene, signal transduction gene, DNA binding and transcription gene etc, 12 showed increased expression including cell receptor gene, immune-associated gene, metabolism-associated gene etc in matrine-treated K562 cells as compared with that in non-treated cell.
CONCLUSIONThe genes, that are closely associated with differentiation of can-cer cell, could be the potential targets for cancer treatment.
