Cell origin of localized Castleman's disease of hyaline-vascular type.
- Author:
Liang-chun WANG
1
;
Ding-fang BU
;
Ping ZHU
;
Xue-jun ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Antigens, CD20; analysis; B-Lymphocytes; metabolism; pathology; Castleman Disease; genetics; metabolism; pathology; Clone Cells; Female; Gene Rearrangement, B-Lymphocyte, Heavy Chain; genetics; Humans; Immunohistochemistry; Leukocyte Common Antigens; analysis; Male; Prognosis; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Hematology 2004;25(7):397-400
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the cell features of 6 Castleman's disease patients and evaluate their prognosis.
METHODSThe resected tumors were investigated by routine histopathology and immunohistochemistry. Reverse-transcript PCR (RT-PCR) and sequencing of RT-PCR products were used to assess the clonal characters of the main tumor cells.
RESULTSHistologically, all of the 6 tumors were classified as the hyaline vascular type. B-cells dominated the follicular germinal centers, with T-cells dispersing inter-follicularly. The results of RT-PCR each obtained a single band of either 128 bp or 122 bp and sequencing showed that there was highly homogeneity within the same length sequences, accompanied by fewer different nucleotide acids.
CONCLUSIONMonoclonal and/or oligoclonal B cells were identified in Castleman's disease. These B cells were originated from germinal center cells.
