A combined assay of multiplex RT-PCR and karyotypic analysis in childhood acute lymphoblastic leukemia.
- Author:
Jun HE
1
;
Yong-quan XUE
;
Jian-qin LI
;
Hai-long HE
;
Ya-xiang HE
;
Yi-ping HUANG
;
Yi-huan CHAI
;
Ling-li ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; Chromosome Aberrations; Female; Humans; Infant; Karyotyping; Male; Oncogene Proteins, Fusion; genetics; Precursor Cell Lymphoblastic Leukemia-Lymphoma; classification; diagnosis; genetics; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; methods; Sensitivity and Specificity
- From: Chinese Journal of Hematology 2004;25(7):413-416
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the value of combination assay of multiplex RT-PCR and karyotypic analysis in the diagnosis and classification of childhood acute lymphoblastic leukemia (ALL).
METHODSFifty cases of childhood ALL patients were studied by multiplex RT-PCR in combination with R or G banding karyotype analysis.
RESULTSOf the 50 childhood ALL patients, 18 (36.0%) carried 11 types of fusion genes including E2A/PBX1, TEL/AML1, TLS/ERG, MLL/AF4, MLL/AF9, MLL/AF10, MLL/AFX, MLL/AF6, MLL/ELL, TAL1D, and HOX11, revealed by multiplex RT-PCR, and in 48 cases, 24 (57.1%) had chromosome abnormalities. Among the latter, numeral chromosome abnormalities and chromosome deletions accounted for 75.0% (18/24), while translocations 25.0% (6/24). The multiplex RT-PCR in combination with chromosome analysis could detect genetic abnormalities in 70% (35/50) of childhood ALL.
CONCLUSIONSMultiplex RT-PCR combined with chromosome analysis can enhance the detection rate of genetic abnormalities in childhood ALL. It provides reliable evidence for the diagnosis, classification and prognosis.
