In vitro effect of interferon alpha on proliferation of human marrow fibroblast.
- Author:
Jin-wen HUANG
1
;
Jing XU
Author Information
- Publication Type:Journal Article
- MeSH: Bone Marrow Cells; cytology; Cell Division; drug effects; Cells, Cultured; Drug Synergism; Fibroblasts; cytology; Humans; Interferon-alpha; pharmacology; Platelet-Derived Growth Factor; pharmacology; Recombinant Proteins; Transforming Growth Factor beta; pharmacology
- From: Chinese Journal of Hematology 2004;25(8):478-481
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of interferon alpha (IFN-alpha) on proliferation of human marrow fibroblasts.
METHODSMarrow fibroblasts were isolated from patients with iron deficiency anemia. Platelet derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1) or INF-alpha2b was added in the cell cultures each alone or in combinations. Cell proliferation and cell cycle of fibroblasts were detected by MTT and flow cytometry, respectively.
RESULTSPDGF-ABqa (1 approximately 50 ng/ml) or TGF-beta1 (0.01 approximately 1 ng/ml) could stimulate the proliferation of the fibroblasts, while PDGF-AB was more effective (P < 0.01). IFN-alpha2b partially suppressed PDGF-AB induced proliferation of fibroblasts,the inhibition rate was 7.62%. IFN-alpha2b and TGF-beta1 combination suppressed synergistically not only the proliferation of the fibroblasts (P < 0.01) but also the PDGF-AB induced-proliferation (P < 0.01) at S phase.
CONCLUSIONIFN-alpha2b combined with TGF-beta1 could synergistically suppress the PDGF-AB induced proliferation of fibroblasts at S phase in vitro.
