Effects of 2A-1-1 on the aggregation and Ca2+ influx of platelets.
- Author:
Fu-ren ZENG
1
;
Song-mei YIN
;
Shuang-feng XIE
;
Da-nian NIE
;
Li-ping MA
;
Jian-hong FENG
;
Li-zhuo XU
;
Yong-yuan GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Diphosphate; pharmacology; Adult; Blood Platelets; cytology; drug effects; metabolism; Calcium; metabolism; pharmacokinetics; Calcium Channel Blockers; pharmacology; Dose-Response Relationship, Drug; Female; Ginsenosides; pharmacology; Humans; Imidazoles; pharmacology; Indoles; pharmacology; Male; Nifedipine; pharmacology; Platelet Aggregation; drug effects; Platelet Aggregation Inhibitors; pharmacology
- From: Chinese Journal of Hematology 2004;25(9):544-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of 2A-1-1 (purified component from Panax notoginsengs saponins) on the aggregation of and Ca2+ influx into human platelets.
METHODSThe aggregation of platelets was tested by nephelometry, Fura-2 fluorescent technique was used for detecting cell [Ca2+]i. The effects of 2A-1-1, nifedipine and SK&F96365 on Ca(2+) influx into human platelets induced by ADP or CPA were observed separately.
RESULTSNifedipine (< 20 micromol/L) could not inhibit platelet aggregation induced by ADP or the Ca(2+) influx induced by ADP or CPA. SK&F96365 at 20 micromol/L could inhibit the maximal aggregation of platelets induced by ADP with a inhibitory rate of 59.83%, at 15 micromol/L could inhibit the Ca2+ influx induced by CPA or ADP. 2A-1-1 (5, 10 and 20 micromol/L) could inhibit the maximal aggregation of platelets induced by ADP with the inhibitory rates of 47.06%, 53.47% and 71.52%, respectively. 2A-1-1 at 10 and 20 micromol/L could inhibit the Ca2+ influx induced by CPA or ADP.
CONCLUSIONS2A-1-1 can inhibit platelets aggregation, block the ROC (Receptor-dependent Ca2+ channels) and inhibit Ca2+ influx of human platelets.
