Screening for virulence strains of Metarhizium against Dorysthenes hydropicus pascoes.
- Author:
Wei-Si MA
1
;
Hai-Li QIAO
1
;
Xiang-Qun NONG
2
;
Jun CHEN
1
;
Jin YU
1
;
Rong-Min QIN
3
;
Chang-Qing XU
1
;
Jiang XU
1
;
Sai LIU
1
;
Xiang-Ming LI
3
;
Hui-Zhen CHENG
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Citrus; parasitology; Coleoptera; microbiology; Metarhizium; growth & development; isolation & purification; pathogenicity; Pest Control, Biological; Plant Diseases; parasitology; prevention & control; Soil Microbiology; Spores, Fungal; growth & development; isolation & purification; pathogenicity; Virulence
- From: China Journal of Chinese Materia Medica 2013;38(20):3438-3441
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe aim of the present study was to screen the Metarhizium strains with high virulence against the larvae of Dorysthenes hydropicus, a serious pest of Citrus grandis.
METHODThirty six strains of Metarhiziums were isolated from the soil of C. grandis GAP base and collected from other institutions, and the pathogenicity of these strains against 1st instar larvae of D. hydropicus was detected at concentration of 1 x 10(8) conidia/g. The high violence strains against D. hydropicus were cultivated in sabouraud dextrose yeast medium at first, then transfer to rice grain. And the sporulations of these violent strains against D. hydropicus were detected.
RESULTTwenty-eight strains showed virulence against D. hydropicus by preliminary study, and 7 strains of them were collected for further study, 6 of the 7 showed high virulence, the highest cadaver rate was higher than 74%. The conidia production of strain 1 and strain 4 were 2.35 +/- 0. 25 (1 x 10(9) conidia/g), 2.21 +/- 0.27 (1 x 10(9) conidia/g), respectively, showed significantly higher than other strains.
CONCLUSIONStrain 1 and strain 4 of the 36 Metarhiziums strains showed high virulence against D. hydropicus, and the highest sporulation ability, so they have a best application prospect.
