Effect of euodiae fructus on hepatic energy metabolism-related mechanisms of reserpine-induced asthenia cold syndrome rats.
- Author:
Li-Ping HUANG
1
;
Ri-Yue YU
2
;
Shu-Hong PENG
2
;
Sen LI
2
;
Yong-Jia ZHAO
2
;
Hong-Ning LIU
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Asthenia; chemically induced; drug therapy; genetics; metabolism; Drugs, Chinese Herbal; administration & dosage; Energy Metabolism; drug effects; Evodia; chemistry; Fruit; chemistry; Humans; Liver; drug effects; metabolism; Male; Rats; Rats, Sprague-Dawley; Reserpine; adverse effects
- From: China Journal of Chinese Materia Medica 2013;38(20):3539-3543
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the effect of Euodiae Fructus on hepatic energy metabolism-related mechanisms of mitochondria of hepatic tissues of asthenia cold syndrome rats.
METHODRats were subcutaneously injected with Reserpine to establish the model. After the oral administration with Euodiae Fructus for 12 d, the oxygen electrode method was adopted to determine the respiration efficiency. The expressions of Cox4, Atp5b, Ucp2,Pgc-1alpha, Nrf1, Tfam mRNA were assayed by using RT-PCR method.
RESULTEuodiae Fructus 4.2 g x kg(-1) could obviously increase ST3 and RCR of asthenia cold syndrome rats, and expressions of Cox4, Ucp2 Nrf1 mRNA. It could also increase expressions of Atp5b and Pgc-1alpha mRNA, but with no statistical significance. No obvious change was observed in Tfam mRNA expression. Euodiae Fructus 4.2 g x kg(-1) could significantly increase ST3 and RCR of asthenia cold syndrome rats and Pgc-1alpha mRNA and Nrf1 mRNA expressions, and significantly decrease P/O, with no obvious impact on Cox4, AtpSb, Ucp2, Tfam mRNA expressions.
CONCLUSIONEuodiae Fructus can promote mitochondrial respiratory function and oxidative phosphorylation efficiency by improving Pgc-1alpha mRNA and Nrf1 mRNA expressions and regulating Cox4 and Atp5b mRNA in mitochondrial respiratory chain. It can also strengthen mitochondrial uncoupling respiration and add heat production by activating Ucp2 mRNA expression in liver.
