Protective effect of succinic acid on primary cardiomyocyte hypoxia/reoxygenation injury.
- Author:
Xi-Lan TANG
1
;
Jian-Xun LIU
2
;
Peng LI
2
;
Wei DONG
2
;
Lei LI
2
;
Yong-Qiu ZHENG
2
;
Jin-Cai HOU
2
Author Information
1. Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China. xilan2013@gmail.com
2. Experimental Research Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
drug effects;
Caspase 3;
metabolism;
Cell Hypoxia;
drug effects;
Cell Survival;
drug effects;
Cells, Cultured;
Female;
Humans;
Hypoxia;
metabolism;
physiopathology;
prevention & control;
Male;
Myocardial Reperfusion Injury;
metabolism;
physiopathology;
prevention & control;
Myocytes, Cardiac;
cytology;
drug effects;
metabolism;
Oxygen;
metabolism;
Protective Agents;
pharmacology;
Proto-Oncogene Proteins c-akt;
metabolism;
Rats;
Rats, Sprague-Dawley;
Succinic Acid;
pharmacology
- From:
China Journal of Chinese Materia Medica
2013;38(21):3742-3746
- CountryChina
- Language:Chinese
-
Abstract:
To establish cardiomyocyte hypoxia/reoxygenation injury model by culturing primary cardiomyocytes from suckling SD rats, in order to study the effect of succinic acid on LDH leakage rate cardiomyocyte ischemia/reperfusion injury. Furthermore, flow cytometry and western blot were conducted to detect the effect of succinic acid on cardiomyocyte apoptosis, cleaved caspase-3 and p-Akt, and discuss the protective effect of succinic acid on primary cardiomyocyte hypoxia/reoxygenation injury of primary cardiomyocytes from neonatal SD rats. According to the findings of the study, succinic acid at the concentrations ranging between 31.25 mg x L(-1) and 500 mg x L(-1) had no significant effect on primary cardiomyocyte activity, and succinic acid at the concentrations of 400, 200, 100, 50 mg x L(-1) could notably reduce cardiomyocyte ischemia/reperfusion LDH leakage rate (P < 0.01 or P < 0.05, respectively). Succinic acid at the concentrations of 400 mg x L(-1) and 200 mg x L(-1) could significantly reduce the percentage of cardiomyocyte apoptosis (P < 0.05), and inhibit the protein expression of cleaved caspase-3 caused by cardiomyocyte ischemia/reperfusion (P < 0.05). Succinic acid at the concentration of 400 mg x L(-1) could remarkably increase the protein expression of cardiomyocyte Akt (P < 0.05), while succinic acid at the concentration of 200 mg x L(-1) had no obvious effect on the protein expression of cardiomyocyte Akt. Therefore, this study demonstrated that succinic acid could inhibit necrosis and apoptosis caused by cardiomyocyte hypoxia/reoxygenation by activating Akt phosphorylation.
