The fabrication of hepatitis B electrochemical immunosensor array.
- Author:
Xiaofeng DAI
1
;
Zhongming LIU
Author Information
1. General Hospital of Guangzhou Command, Guangzhou 510010, China.
- Publication Type:Journal Article
- MeSH:
Biosensing Techniques;
instrumentation;
Electric Impedance;
Electrochemistry;
instrumentation;
methods;
Electrodes;
Hepatitis B Antibodies;
immunology;
Hepatitis B Surface Antigens;
analysis;
immunology;
Humans;
Immunoassay;
instrumentation;
methods
- From:
Journal of Biomedical Engineering
2008;25(2):450-453
- CountryChina
- Language:Chinese
-
Abstract:
Based on integrate circuit (IC) technology, an eight-channel gold electrodes (GEs) array was developed. The immunosensor array is prepared by co-immobilizing thionine and Hepatitis B (HB) antibody on the gold electrodes through covalently binding them to GEs with a cysteamine/glutaraldehyde linkage. Hepatitis B surface antigen (HBsAg) was detected qualitatively and quantitatively by the peak current decrease percentage of the thionine. HBsAg positive/negative standard serum was well defined by the array. 8-channel synchronous detection for HBsAg was noted to be of good accuracy and reliability. The results of its clinical application were in good agreement with the results from ELISA.
