Surface modification of biodegradable polymer/TCP scaffolds and related research.
- Author:
Xing MA
1
;
Yunyu HU
;
Xiaoming WU
;
Yongnian YAN
;
Zhuo XIONG
;
Rong LU
;
Jun WANG
;
Dan LI
;
Xinzhi XU
Author Information
1. Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi 'an 710032, China. maping@fmmu.edu.cn
- Publication Type:Journal Article
- MeSH:
Biocompatible Materials;
chemistry;
Bone Morphogenetic Proteins;
chemistry;
Bone Substitutes;
chemistry;
Calcium Phosphates;
chemistry;
Humans;
Lactic Acid;
chemistry;
Microscopy, Electron, Scanning;
Polyglycolic Acid;
chemistry;
Porosity;
Surface Properties;
Tissue Engineering;
methods
- From:
Journal of Biomedical Engineering
2008;25(3):571-577
- CountryChina
- Language:Chinese
-
Abstract:
Under laboratory condition, the compound materials of Poly (DL-lactic-co-glycolic acid)/Tricalcium phosphate [PLGA/TCP(L), with component ratio of 7:3] were fabricated by combining the thermally induced phase separation (TIPS) with solvent-casting particulate-leaching (SCPL) approach. On the other hand, rapid prototyping (RP) technique manufactured PLGA/TCP scaffolds [PLGA/TCP(RP)] were obtained. These two kinds of carriers were coated with collagen type I (Col I). The extracted bovine bone morphogenetic protein (bBMP) was loaded into carriers to establish biomimetic synthetic bones. PLGA/TCP(L) scaffolds, demineralized bone matrices (DBM) of bovine cancellous bone, PLGA/TCP(L) scaffolds, biomimetic synthetic bones and OsteoSet bone graft substitutes were investigated. Scanning electron microscopy revealed that the microarchitecture of PLGA/TCP(RP) scaffolds was much better than that of PLGA/TCP(L) scaffolds. The diameter of macropore of PLGA/TCP(RP) scaffold was 350 microm. The porosities of PLGA/ TCP(L) scaffolds, DBM, PLGA/TCP(RP) scaffolds and OsteoSet bone graft substitutes were 21.5%, 70.4%, 58.6% and 0%, respectively (P<0.01). Modification of PLGA/TCP scaffolds with collagen type I [PLGA/TCP(L)-Col I and PLGA/TCP(RP)-Col I] essentially increased the affinity of the carriers to bBMP. Among these synthetic materials, PLGA/TCP(RP)-Col I-bBMP composite is promising as a novel bone graft substitute due to its advanced fabrication technique, good tri-dimensional microarchitecture and ideal components.
