DNA microarray reveals changes in gene expression of endothelial cells under shear stress.
- Author:
Min CHENG
1
;
Wensheng ZHANG
;
Huaiqing CHEN
;
Wenchao WU
;
Hua HUANG
Author Information
1. Institute of Biomedical Engineer, West China Medical Center of Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Cell Division;
genetics;
Cells, Cultured;
Endothelium, Vascular;
cytology;
metabolism;
Gene Expression Profiling;
Humans;
Oligonucleotide Array Sequence Analysis;
RNA, Messenger;
biosynthesis;
genetics;
Stress, Mechanical;
Umbilical Veins;
cytology;
metabolism
- From:
Journal of Biomedical Engineering
2004;21(2):188-192
- CountryChina
- Language:Chinese
-
Abstract:
cDNA microarray technology is used as a powerful tool for rapid, comprehensive, and quantitative analysis of gene profiles of cultured human umbilical vein endothelial cells(HUVECs) in the normal static group and the shear stressed (4.20 dyne/cm2, 2 h) group. The total RNA from normal static cultured HUVECs was labeled by Cy3-dCTP, and total RNA of HUVECs from the paired shear stressed experiment was labeled by Cy5-dCTP. The expression ratios reported are the average from the two separate experiments. After bioinformatics analysis, we identified a total of 108 genes (approximately 0.026%) revealing differential expression. Of these 53 genes expressions were up-regulated, the most enhanced ones being human homolog of yeast IPP isomerase, human low density lipoprotein receptor gene, Squalene epoxidase gene, 7-dehydrocholesterol reductase, and 55 were down-regulated, the most decreased ones being heat shock 70 kD protein 1, TCB gene encoding cytosolic thyroid hormone-binding protein in HUVECs exposed to low shear stress. These results indicate that the cDNA microarray technique is effective in screening the differentially expressed genes in endothelial cells induced by various experimental conditions and the data may serve as stimuli to further researches.
