Expression and purification of mouse B lymphocyte chemoattractant.
- Author:
Yu JIANG
1
;
Yanjun WEN
;
Jiyan LIU
;
Chunhua ZOU
;
Ling TIANG
;
Yuquan WEI
Author Information
1. Key Laboratory of Biotherapy of Human Diseases of Ministry of National Education, Cancer Center, West China Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Chemokine CXCL13;
Chemokines, CXC;
biosynthesis;
genetics;
isolation & purification;
Escherichia coli;
genetics;
Genetic Vectors;
Mice;
Mice, Inbred BALB C;
Plasmids;
genetics;
Prokaryotic Cells;
metabolism;
Recombinant Proteins;
biosynthesis;
genetics;
isolation & purification;
Sequence Analysis, DNA
- From:
Journal of Biomedical Engineering
2004;21(2):251-254
- CountryChina
- Language:Chinese
-
Abstract:
A DNA fragment encoding mouse B Lymphocyte Chemoattractant (BLC, MV10Kda) was obtained by PCR. The amplified fragment was inserted into prokaryotic expression vector PQE30. Recombinant protein was expressed in E. Coli XL-1 blue and purified by affinity chromatography on a nickel-nitrilotriacetic acid gel matrix. Then it was identified by sequence analysis and Western blot analysis. The fragment inserted into prokaryotic expression vector PQE30 was identified to be BLC gene fragment by sequence analysis. And a specfic band was shown by Western blot analysis. These findings provide the evidence that the recombinant protein obtained and purified in this study using gene engineering method is mouse B Lymphocyte Chemoattractant.
