Construction of the recombinant adenovirus vector carrying antisense multidrug resistance (MDR1) gene.
- Author:
Bo LI
1
;
Xinghua GOU
;
Lin CHEN
;
Lünan YAN
;
Dehua LI
;
Yongheng ZHAO
;
Le HAN
;
Lanying ZHAO
Author Information
1. Department of General Surgery, West China Hospital Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
biosynthesis;
genetics;
Adenoviridae;
genetics;
metabolism;
Carcinoma, Hepatocellular;
drug therapy;
genetics;
Cell Line, Tumor;
Drug Resistance, Neoplasm;
genetics;
Escherichia coli;
genetics;
Gene Transfer Techniques;
Genes, MDR;
genetics;
Genetic Vectors;
Humans;
Liver Neoplasms;
drug therapy;
genetics;
Multidrug Resistance-Associated Proteins;
biosynthesis;
genetics;
Oligonucleotides, Antisense;
pharmacology;
Plasmids;
Recombinant Proteins;
biosynthesis;
genetics;
Recombination, Genetic
- From:
Journal of Biomedical Engineering
2004;21(3):424-427
- CountryChina
- Language:Chinese
-
Abstract:
The fragment of MDR1 gene obtained from the plasmid pHaMDR1-1 carrying the whole human MDR1 cDNA, was cloned reversely into the shuttle plasmid pAdTrack-CMV. With the resultant plasmid and the backbone plasmid pAdEasy-1, the homologous recombination took place in the Escherichia coli BJ5183 and the recombinant adenoviral plasmid was generated. The adenoviruses were packaged in the 293 cells. The recombinant adenovirus MDR1 vector would introduce the antisense MDR1 gene into the human multidrug resistance hepatocellular cell line effectively, which would provide an experimental basis for studies on the multidrug resistance in human hepatocellular carcinoma.
