Identification of differentially expressed genes of acute hypoxia-treated HepG2 cells and hypoxia-acclimatized HepG2 cells.
- Author:
Jin-Hui WANG
1
;
Ya-Jun SHAN
;
Yu-Wen CONG
;
Lan-Jun WU
;
Xiao-Ling YUAN
;
Zhen-Hu ZHAO
;
Sheng-Qi WANG
;
Jia-Pei CHEN
Author Information
1. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850. wangjinhui20@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Adaptation, Physiological;
genetics;
physiology;
Cell Hypoxia;
genetics;
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic;
Hep G2 Cells;
Humans;
Nucleic Acid Hybridization;
methods;
Oxygen;
metabolism;
Transcriptome
- From:
Acta Physiologica Sinica
2003;55(3):324-330
- CountryChina
- Language:Chinese
-
Abstract:
To provide necessary information for further understanding of molecular mechanism of hypoxia acclimatization, the differentially expressed genes of HepG2 cells exposed to normoxia, acute hypoxia-treated cells which were exposed to 1% oxygen for 48 h, and hypoxia-acclimatized HepG2 cells which were cultured for 6 circles of alternate low oxygen (1% oxygen for 24 h) and normal oxygen (21% oxygen for 24 h), were identified respectively by combining the suppression subtractive hybridization (SSH) and cDNA microarray. Thirty-seven genes were expressed differentially in cells exposed to 1% oxygen for 48 h compared with those in cells exposed to normoxia. The expression of all these 37 genes was down-regulated, including the genes participating in cell cycle, cell response to stimulus, and cell signal transduction, and cell cytoskeleton formation, the genes associated with transcription and cell metabolism, 4 expressed sequence tags (ESTs), and 12 genes of which the functions are not known. There is a novel gene sequence, which has not been found in existing databases. There were only 6 genes differentially expressed in the hypoxia-acclimatized cells compared with cells exposed to normoxia, including two mitochondrion genes, metalloprotease-1 gene, ferritin gene, thymosin beta-4 and TPT1 genes. The expressions of mitochondrion ND4, ferritin, thymosin beta-4 and TPT1 were up-regulated, while the expressions of mitochondrion ND1 gene and metalloproease-1 gene were down-regulated. Cell tolerance to hypoxia increased after the cells were hypoxia-acclimatized. The different gene expression patterns of the acute hypoxia-treated cells and the hypoxia-acclimatized cells may be related to the increased tolerance of the cells to hypoxia.
