Differentially expressed genes associated with cold acclimation.
- Author:
Fa-Qing YANG
1
;
Ling-Jia QIAN
;
Wan-Yin WANG
;
Hui-Rong REN
;
Da XU
Author Information
1. Institute of Hygiene and Environment Medicine, Tianjin 300050.
- Publication Type:Journal Article
- MeSH:
Acclimatization;
genetics;
Animals;
Cold Temperature;
Gene Expression;
Liver;
metabolism;
Male;
Mice;
Mice, Inbred BALB C;
Muscle, Skeletal;
metabolism;
Transcriptome;
Up-Regulation
- From:
Acta Physiologica Sinica
2003;55(3):360-363
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the upregulated genes associated with cold acclimation, a cold acclimation model was established based on Balb/C mouse. mRNA of muscle and liver were isolated, and the upregulated genes of these tissues were studied by representational differential analysis (RDA). The upregulated genes then were sequenced and searched by Blast software in GenBank database. The results showed that some genes were upregulated and possibly associated with cold acclimation. Three of these genes, transferrin, fibrinogen B-beta-chains and a new gene fragment (Genbank ID: AF454762), were confirmed to be upregulated by RNA slot-blot analysis. The finding of these genes might contribute to further understanding of the molecular mechanisms of cold acclimation.
