Arginine vasopressin-induced nitric oxide content changes in cultured cardiac fibroblasts and its relation to nuclear factor-kappaB.
- Author:
Yan-Hong FAN
1
;
Lian-You ZHAO
;
Qiang-Sun ZHENG
;
Yu-Sheng XUE
;
Xue-Dong YANG
;
Jian-Wei TIAN
;
Lin XU
Author Information
1. Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi an 710033.
- Publication Type:Journal Article
- MeSH:
Animals;
Animals, Newborn;
Arginine Vasopressin;
pharmacology;
Cells, Cultured;
Fibroblasts;
cytology;
metabolism;
Myocytes, Cardiac;
cytology;
metabolism;
NF-kappa B;
metabolism;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase Type II;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Rats;
Rats, Sprague-Dawley
- From:
Acta Physiologica Sinica
2003;55(4):417-421
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the changes in the nitric oxide (NO) contents, nitric oxide synthase (NOS) activity and inducible nitric oxide (iNOS) mRNA expression in arginine vasopressin (AVP)-induced cardiac fibroblasts (CFs) in vitro and its relation to nuclear factor-kappaB (NF-kappaB), CFs were isolated by trypsin digestion method. Nitric acid reductase method, spectrophotometry, reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence-interactive laser cytometer techniques and Western blotting were used respectively to detect NO contents, NOS activity, iNOS mRNA expression and the activation of NF-kappaB in CFs. AVP increased NO contents, NOS activity and iNOS mRNA expressions in a concentration-dependent manner; NF-kappaB was activated and mobilized from cytoplasm to nucleus in AVP-induced CFs; PDTC, one of the inhibitors of NF-kappaB, could inhibit aforementioned increments. It is suggested that the increases in NO contents, elevation of NOS activity and increment of iNOS mRNA expression may be mediated through NF-kappaB activation pathway in cultured CFs induced by AVP, and that NF-kappaB is involved in the occurrence and development of myocardial fibrosis.
