Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro.
- Author:
Xiao-Ping CHEN
1
;
Hong LIU
;
Shu-Hong LIU
;
Yan WU
;
Hai-Tao WU
;
Ming FAN
Author Information
1. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850.
- Publication Type:Journal Article
- MeSH:
Adult;
Cell Differentiation;
drug effects;
Cell Proliferation;
Cells, Cultured;
Ciliary Neurotrophic Factor;
pharmacology;
Humans;
Middle Aged;
Muscle, Skeletal;
cytology;
Myoblasts;
cytology;
Recombinant Proteins;
pharmacology;
Young Adult
- From:
Acta Physiologica Sinica
2003;55(4):464-468
- CountryChina
- Language:Chinese
-
Abstract:
To determine the role of recombinant human ciliary neurotrophic factor (rhCNTF) in myogenesis, we observed the effects of rhCNTF (0 10 ng/ml) on myoblast differentiation of adult human in vitro. The results showed that compared with the control group, the groups of 2.5 10 ng/ml rhCNTF treatment significantly inhibited myoblast differentiation (P<0.01), and the inhibition was dose-dependent and reversible. Western blots also indicated that compared with the control group, the expression of myogenin and p21, markers of myoblast differentiation phase, was significantly reduced (P<0.01), while Myf5 and desmin, markers of myoblast proliferative phase, significantly increased (P<0.01) in the groups of 2.5 10 ng/ml rhCNTF treatment. These findings demonstrate that exogenous rhCNTF can reversibly inhibit differentiation but permits proliferation of adult human myoblasts in vitro.
