Synergistic regulation of the acute phase protein SIP24/24p3 by glucocorticoid and pro-inflammatory cytokines.
- Author:
Quan-Sheng LIU
1
;
Marit NILSEN-HAMILTON
;
Si-Dong XIONG
Author Information
1. Department of Immunology and Key Laboratory of Molecular Medicine of Ministry of Education, Shanghai Medical College of Fudan University.
- Publication Type:Journal Article
- MeSH:
Acute-Phase Proteins;
biosynthesis;
genetics;
Animals;
BALB 3T3 Cells;
Carrier Proteins;
biosynthesis;
genetics;
Cytokines;
pharmacology;
Dexamethasone;
pharmacology;
Drug Synergism;
Gene Expression Regulation;
Interleukin-6;
pharmacology;
Lipocalin-2;
Lipocalins;
Mice;
Mice, Inbred BALB C;
Oncogene Proteins;
biosynthesis;
genetics;
RNA, Messenger;
biosynthesis;
genetics;
Tumor Necrosis Factor-alpha;
pharmacology
- From:
Acta Physiologica Sinica
2003;55(5):525-529
- CountryChina
- Language:English
-
Abstract:
SIP24/24p3 is a secreted murine acute phase protein which has been speculated to play an anti-inflammatory role in vivo. Recently SIP24/24p3 has been found to be able to specifically induce apoptosis in leukocytes. By using (35)S metabolic labeling method, we studied the regulation of SIP24/24p3 by glucocorticoid and pro-inflammatory cytokines IL-6 and TNF-alpha in cultured Balb/c 3T3 and BNL cells. The following results were observed: (1) dexamethasone induced the expression of SIP24/24p3 in both Balb/c 3T3 and BNL cells, the induction was more significant in BNL cells; (2) dexamethasone and IL-6 synergistically induced the expression of SIP24/24p3 in both Balb/c 3T3 and BNL cells; (3) in Balb/c 3T3 cells dexamethasone and TNF-alpha acted synergistically to induce the expression of SIP24/24p3, whereas in BNL cells dexamethasone and TNF-alpha induced the expression of SIP24/24p3 in an additive manner; (4) dexamethasone and IL-6/TNF-alpha acted synergistically in Balb/c 3T3 cells and additively in BNL cells to induce the expression of SIP24/24p3. The inducibility of SIP24/24p3 by multiple factors will help to explain its highly specific expression in vivo. The difference in the expression patterns of SIP24/24p3 in different cell types is also suggestive to its expression and regulation in hepatic and extrahepatic tissues. Finally, the fact that SIP24/24p3 protein can be induced by both pro-inflammatory as well as anti-inflammatory factors is indicative of the important role of SIP24/24p3 in the entire acute phase response process.
