Tissue-specific Expression of DNA Repair Gene, N-Methylpurine-DNA Glycosylase (MPG) in Balb/c Mice without External Damage.
- Author:
Nam Keun KIM
1
Author Information
1. Human Genetics Center, CHA General Hospital College of Medicine, Pochon CHA University, Korea.
- Publication Type:Original Article
- Keywords:
DNA repair;
gene expression;
mouse;
MPG;
tissue-specificity
- MeSH:
Adult;
Animals;
DNA Repair*;
DNA*;
Gene Expression;
Humans;
Infant, Newborn;
Mice*;
Postpartum Period;
Purines;
RNA, Messenger;
Testis;
Thymus Gland
- From:Journal of Genetic Medicine
1998;2(1):31-34
- CountryRepublic of Korea
- Language:English
-
Abstract:
The N-methylpurine-DNA glycosylase (MPG), a ubiquitous DNA repair enzyme, removes N-methylpurine and other damaged purines induced in DNA. Tissue-specific mRNA levels of the N-methylpurine-DNA glycosylase (MPG) were investigated in Balb/c mice of four different growing stages; newborn, 1, 4 and 8-weeks postpartum. MPG expressions in the newborn and the 8-week-old mice were the highest in thymus and testis, respectively. The tested tissues of the newborn mice had consistently higher MPG mRNA level than 8-week-old adults except in testis and thymus. The MPG mRNA level in testis was the lowest in the newborn mice, but it attained the highest in the 8-week-old mice. The levels of MPG mRNA among the different tissues in the newborn and the 8-week-old mice were more than 9.0 and 19.0-fold respectively. These results suggest that the of MPG expression was dependent on the growing stage and had tissue-specificity.
