Role of calcineurin in the progression of cardiac hypertrophy in renovascular hypertensive rats.
- Author:
Hong-Zhuan SHENG
1
;
Ji-Nan ZHANG
;
Di YANG
;
Guo-Ping YANG
;
Jin-Dan XU
;
Xiang-Jian CHEN
Author Information
1. Institute of Cardiovascular Disease Research, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029.
- Publication Type:Journal Article
- MeSH:
Animals;
Calcineurin;
biosynthesis;
genetics;
metabolism;
Cyclosporine;
pharmacology;
Hypertension, Renovascular;
complications;
metabolism;
physiopathology;
Hypertrophy, Left Ventricular;
etiology;
metabolism;
physiopathology;
RNA, Messenger;
biosynthesis;
genetics;
Rats;
Rats, Sprague-Dawley
- From:
Acta Physiologica Sinica
2003;55(5):594-598
- CountryChina
- Language:Chinese
-
Abstract:
The present study was to investigate the mRNA, protein expression and the activity of calcineurin in the hypertrophic heart, and to determine the effect of calcineurin inhibitor--cyclosporine A (CsA) on the regression of cardiac hypertrophy in renovascular hypertensive rats. Renovascular hypertension was induced by two kidney-one clip methods. Two months after the operation, cardiac hypertrophy was determined by histological analysis performed in some rats (2K1C-2M), then the rats were subdivided into 2 groups: (1) 3-month old two kidney-one clip group (2K1C-3M) with rats receiving 0.9% NaCl per day for one month, and (2) CsA-treated group with rats treated with CsA for one month. Sham-operated rats were used as control. The ratio of the left ventricular weight to tibial length (LVW/TL), the area of cardiac myocyte, mRNA and protein expression and the activity of calcineurin were determined. Both the LVW/TL and the cardiomyocyte area were significantly larger in 2K1C-2M and 2K1C-3M rats than in age-matched sham-operated rats. Treatment with CsA significantly attenuated the increase in the LVW/TL as well as the cardiomyocyte area. The mRNA, protein expression and the activity of calcineurin were significantly higher in 2K1C-2M and 2K1C-3M rats than those in the age-matched sham-operated rats, while the elevation of mRNA, protein expression and activity of calcineurin were significantly suppressed in the CsA-treated rats. In conclusion, calcineurin plays a role in the progression of cardiac hypertrophy in renovascular hypertensive rats. The inhibition of calcineurin can reverse cardiac hypertrophy.
