Differential susceptibility of naïve versus cloned CD4+ T cells to antigen-specific and MHC-restricted anergy induction.
- Author:
Quan-Sheng LIU
1
;
Rui-Hua ZHANG
;
Yi-Wei CHU
;
Si-Dong XIONG
Author Information
1. Department of Immunology of Shanghai Medical College of Fudan University, Key Laboratory of Molecular Medicine of Ministry of Education, Shanghai Gene Immunization Vaccine Research Center, Shanghai 200032.
- Publication Type:Journal Article
- MeSH:
Animals;
Antigen-Presenting Cells;
immunology;
metabolism;
Antigens, CD;
genetics;
immunology;
metabolism;
CD4 Antigens;
immunology;
CD4-Positive T-Lymphocytes;
cytology;
immunology;
Clonal Anergy;
genetics;
immunology;
Clone Cells;
immunology;
Epitopes, T-Lymphocyte;
biosynthesis;
Immune Tolerance;
genetics;
Major Histocompatibility Complex;
immunology;
Mice;
Mice, Transgenic;
Receptors, Antigen, T-Cell;
physiology
- From:
Acta Physiologica Sinica
2003;55(6):633-640
- CountryChina
- Language:English
-
Abstract:
T cell anergy has been successfully induced under different conditions in cloned CD4(+) T cells, but induction of T cell anergy in vivo has been difficult and controversial. Due to the low frequency of naturally occurring T cell population with specificity to a defined antigen, it is very difficult to study anergy of naïve T cells without prior in vivo priming which complicates the interpretation of experimental data. To solve this problem, we adopted the HNT-TCR transgenic mice which have homogeneous antigen specific CD4(+) T cell population. In this study, we generated an influenza virus hemagglutinin (HA) peptide-specific CD4(+) T cell clone from the HNT-TCR transgenic mice and induced anergy using APCs which were treated with the crosslinker, ECDI (1-ethyl-3-3(3-dimethylaminopropyl) carbodiimide). The proliferative response of the cloned or freshly purified naïve CD4(+) transgenic T cells after treatment with ECDI-treated APCs and the HA peptide antigen was monitored as the index of anergy induction. The results showed that anergy was successfully induced in the cloned HNT-TCR transgenic CD4(+) T cells. It was determined that the induced anergy was antigen- and MHC-specific. By contrast, anergy was not observed in freshly purified naïve CD4(+) transgenic T cells under the same conditions. The results suggest that naïve CD4(+) T cells may have different anergy inducing requirements, or that cloned CD4(+) T cells may have certain priming or in vitro cloning artifact which makes them more susceptible to anergy induction. We propose that induction of T cell anergy may depend on the T cell growth, activation and differentiation state or cloning conditions. The results from the present study may have important implications for the study of the mechanism(s) underlying T cell anergy induction in vivo and for applications of immune tolerance based therapy.
