Anti-tumor effect of adenovirus-mediated Bcl-XL shRNA in vitro.
- Author:
Yu-ping ZHU
1
;
De-chuan LI
;
Hai-yang FENG
;
Yong LIU
;
Jun QIAN
;
Yin-bo CHEN
;
Yun GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Cell Line, Tumor; Cell Proliferation; Colonic Neoplasms; metabolism; pathology; Humans; RNA, Small Interfering; genetics; bcl-X Protein; genetics; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2012;15(3):292-294
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anti-tumor effect of adenovirus-mediated Bcl-XL shRNA on colon cancer cells in vitro.
METHODSA recombinant Bcl-xl adenovirus was constructed, amplified, and purified. The effect on mRNA expression of Bcl-XL was assessed by RT-PCR, and the effect on apoptosis-induction of colon cancer(Lovo cell line) in vitro was assessed by MTT assay and cell clonogenic assay.
RESULTSRT-PCR showed that Ad/Bcl-XL shRNA significantly down-regulated the mRNA expression of Bcl-XL in Lovo cells. Ad/Bcl-XL shRNA suppressed the proliferation of Lovo cells in a dose-dependent as well as a time-dependent manner compared with Ad/GFP (P<0.05). Treatment with Ad/Bcl-XL shRNA dramatically suppressed the colony formation of Lovo cells in a dose-dependent manner (P<0.05). Ad/Bcl-XL shRNA showed no effect on normal human fibroblast.
CONCLUSIONAd/Bcl-XL shRNA exhibits cytotoxic effect on Lovo cells and may have the potential value in the treatment of colon cancer.
