Association of the IL-18 gene polymorphism with susceptibility to colorectal cancer.
- Author:
Jun-yu GUO
1
;
An-qiang QIN
;
Ru-kun LI
;
Chang-mou YANG
;
Fu-da HUANG
;
Zhan-yi HUANG
;
Hou-ji GUO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Case-Control Studies; Colorectal Neoplasms; genetics; Female; Genetic Predisposition to Disease; Haplotypes; Humans; Interleukin-18; genetics; Male; Middle Aged; Polymorphism, Restriction Fragment Length; Polymorphism, Single Nucleotide
- From: Chinese Journal of Gastrointestinal Surgery 2012;15(4):400-403
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate single nucleotide polymorphisms(SNPs) and haplotypes of interleukin-18(IL-18) gene associated with the susceptibility to colorectal cancer(CRC).
METHODSTwo SNPs of IL-18 gene promoter -137G/C and -607C/A in 170 patients with CRC and 160 healthy controls matched by age and sex in a Chinese population were analyzed using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) strategy. Frequency of haplotypes and linkage disequilibrium of IL-18 gene in different groups were analyzed by SHEsis programs.
RESULTSThe distributions of IL-18 gene -607C/A polymorphism did not differ between CRC patients and healthy controls, but IL-18 gene -137G/C polymorphism was significantly different(P<0.05). The relative risk of C allele for CRC was 1.814 times of the G allele (OR=1.814,95% CI:1.246-2.642). Consistent with the results of the genotyping analyses, IL-18 -137G/C and -607C/A polymorphisms showed strong linkage disequilibrium(|D'|=0.945), frequency of the -137C/-607A haplotype in patients with CRC was significantly higher than that in healthy controls(P<0.05). The -137C/-607A haplotype was associated with a significantly increased risk of CRC(OR=1.637, 95% CI:1.100-2.437).
CONCLUSIONSIL-18 gene -137G/C polymorphism and -137C/-607A haplotype are associated with CRC. -137C allele may be an important genetic susceptibility gene for CRC.
