The rescue effect of RANKL on zoledronate induced acid inhibition of osteoclastogenesis and gene expression of NF-kappaB p50 and c-Jun.
- Author:
Chunfeng XU
;
Peng LI
;
Shiyu DING
;
Ren LI
;
Mengchun QI
;
Jinyuan LI
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Resorption;
drug therapy;
Cell Line;
Diphosphonates;
pharmacology;
Gene Expression;
Imidazoles;
pharmacology;
Mice;
NF-kappa B p50 Subunit;
metabolism;
Osteoblasts;
drug effects;
Osteoclasts;
drug effects;
Proto-Oncogene Proteins c-jun;
metabolism;
RANK Ligand;
pharmacology
- From:
Journal of Biomedical Engineering
2014;31(2):385-399
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the rescue effect of receptor activator for nuclear factor-kappaB ligand (RANKL) on zoledronate acid (ZOL) induced inhibition of osteoclastogenesis and gene expression of NF-kappaB p50 and c-Jun was investigated. Mice calvarial osteoblasts (OBs) were harvested and co-cultured with RAW264.7 cells and the cells were divided into 4 groups and received treatment with ZOL and RANKL, either single or combined. The formation of multi-nucleated osteoclast (OC) was examined and gene expression of NF-kappaB p50 and c-Jun was detected. Group B (ZOL) showed least multi-nucleated OC and resorption lacunae among the 4 groups (P < 0.05 or P < 0.01) and it was followed by group C (ZOL+RANKL). Group D (RANKL) showed highest OC and resorption lacunae while it was similar to Group A (control) (P > 0.05). Gene expression of NF-kappaB p50 and c-Jun was the lowest in group B (P < 0.05 or P < 0.01) among the four groups and was significantly increased in group C when compared with group B (P < 0.05). Group A and D showed highest gene expression and they were similar to each other (P > 0.05). This study suggest that RANKL might partly rescue ZOL induced inhibition of osteoclastogenesis, and the effect of RANKL and ZOL on osteoclastogenesis may be mediated by NF-kappaB p50 and c-Jun.
