Effects of Survivin-T34A mutant on breast cancer cell in vitro and in vivo.
- Author:
Xiaohui DENG
;
Haiyan SONG
;
Mingxin REN
- Publication Type:Journal Article
- MeSH:
Animals;
Apoptosis;
Breast Neoplasms;
drug therapy;
pathology;
Humans;
Inhibitor of Apoptosis Proteins;
genetics;
pharmacology;
Injections, Intralesional;
Mice;
Mice, Nude;
Neoplasm Transplantation;
Transfection
- From:
Journal of Biomedical Engineering
2014;31(3):648-651
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of the proliferation and apoptosis of Survivin-T34A mutant on breast cancer MCF-7 cell, we adopted the method of cell culture in vitro to observe the proliferation and apoptosis of the cell. In the experiment, MCF-7 cells were randomly divided into three groups and transfected with normal saline, PORF-9-null and Survivin-T34A, respectively. Breast cancer nude mouse models were established to study anti-tumor effect of Survivin-T34A in vivo. The activity of the cells in the Survivin-T34A-transfected group was lower than that in PORF-9-null group. The increase of cell apoptosis was observed under electron microscopy, meanwhile the apoptotic rate was obviously higher than that in PORF-9-null control by flow cytometry. Tumor inhibition effects of the mouse which received the injection of Survivin-T34A intratumoral injection were apparent, and the inhibition ratio was as high as 47.1%. In conclusion, Survivin-T34A mutant has anti-tumor effect through efficiently inhibiting the growth of breast cancer MCF-7 cell and actively promoting apoptosis of cancer cells.
