RNA Interference in Functional Genomics and Medicine.
10.3346/jkms.2003.18.3.309
- Author:
V Narry KIM
1
Author Information
1. Institute of Molecular Biology and Genetics and School of Biological Science, Seoul National University, Seoul, Korea. narrykim@snu.ac.kr
- Publication Type:Review
- Keywords:
RNA Interference (RNAi);
Gene therapy;
RNA Small interfering (siRNA);
MicroRNAs (miRNA);
Small hairpin RNA (shRNA);
mRNA degradation;
Translation;
Functional genomics
- MeSH:
Animals;
Gene Therapy/*methods/trends;
*Genomics;
Human;
*RNA Interference
- From:Journal of Korean Medical Science
2003;18(3):309-318
- CountryRepublic of Korea
- Language:English
-
Abstract:
RNA interference (RNAi) is the sequence-specific gene silencing induced by double-stranded RNA (dsRNA). Being a highly specific and efficient knockdown technique, RNAi not only provides a powerful tool for functional genomics but also holds a promise for gene therapy. The key player in RNAi is small RNA (~22-nt) termed siRNA. Small RNAs are involved not only in RNAi but also in basic cellular processes, such as developmental control and heterochromatin formation. The interesting biology as well as the remarkable technical value has been drawing widespread attention to this exciting new field.
