Mutation analysis of IgVH gene in B-cell chronic lymphocytic leukemia.
- Author:
Feng WANG
1
;
Huifen ZHU
;
Lijuan ZHU
;
Botao YIN
;
Guanxin SHEN
Author Information
1. Institute of Immunology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Gene Rearrangement;
Humans;
Immunoglobulin Variable Region;
genetics;
Leukemia, Lymphocytic, Chronic, B-Cell;
genetics;
Molecular Sequence Data;
Mutation
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(3):177-182
- CountryChina
- Language:English
-
Abstract:
The variable heavy chain region (VH) genes of 3 untreated patients with B-cell chronic lymphocytic leukemia (B-CLL) were cloned and analyzed. The VH family used was VH3-11, VH3-72 and VH3-33. More than 2% difference from the corresponding germline gene was detected in all the 3 obtained potential functional genes (average 16.7). Mutation pattern analysis indicated evidence of antigen selective pressure observed in 1 of 3 cases. Our findings suggested that the tumor cells originate from post-GC cells.
