Reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring.
- Author:
Jianli GUO
1
;
Yaliang SUN
Author Information
1. Department of Biochemistry, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Ethidium;
pharmacology;
toxicity;
Genetic Variation;
Luciferases;
biosynthesis;
Luminescent Measurements;
Mitomycins;
pharmacology;
toxicity;
Mutagens;
Mutation;
drug effects;
Photobacterium;
genetics;
Toxicology;
methods;
Transcription, Genetic;
drug effects
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(3):180-182
- CountryChina
- Language:English
-
Abstract:
The luminous intensity of dark variant (S1) separated from photobacterium phosphoreum (A2) was 1/10,000 less than that of wild-type. Ethidium bromide (EB) (0.6 mg/L), Mytomycin C (MC, 0.05 mg/L), 2-amino fluorene (2-AF, 1.0 mg/L) all could strongly induce reversion mutation for S1 within 24 h and increase reversion ratio significantly. The results of experiments indicated that these revertants had stable genetic characteristic and the mutation may take place at gene levels. The mutagenesis to S1 caused by EB, MC and 2-AF was detected and it may be used as a new rapid, simple and sensitive method for gene toxicant monitoring.
