Effect of antisense oligonucleotide to annexin II on the t-PA-mediated plasminogen activation in vitro.
- Author:
Xiaohui ZHANG
1
;
Huarong ZHOU
;
Linghui XIA
;
Guanxin SHEN
;
Yu HU
;
Wenning WEI
;
Shanjun SONG
Author Information
1. Institute of Hematology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Annexin A2;
genetics;
metabolism;
physiology;
DNA, Complementary;
genetics;
Endothelium, Vascular;
cytology;
HL-60 Cells;
pathology;
Humans;
Oligonucleotides, Antisense;
genetics;
metabolism;
Plasminogen;
metabolism;
RNA, Messenger;
genetics;
metabolism;
physiology;
Receptors, Cell Surface;
metabolism;
Recombinant Proteins;
genetics;
metabolism;
Tissue Plasminogen Activator;
metabolism;
Transfection;
Umbilical Veins;
cytology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(3):183-185
- CountryChina
- Language:English
-
Abstract:
In order to study the role of annexin II, a recombinant expression vector, pZeoSV2(+) ANN II, containing the annexin II cDNA, was developed. The 1.1-kb-length annexin II cDNA was inserted into a expression vector, PZeoSV(+) and transfected into HL-60 cells which had low baseline expression of Ann- II. pZeoSV(+) ANN II was analyzed by restriction mapping and the Ann- II sequence identified. The ability of the transfected cells, non-transfected and mock-transfected cells to stimulate t-PA-depend plasminogen activation was compared. The results showed that HL-60 with pZeoSV(+) ANN II transfection could significantly increase the plasminogen activation (8.9 +/- 1.2 U) in vitro with the difference being significant as compared with non-transfected (1.5 +/- 0.4 U) and mock-transfected cells (4.2 +/- 0.9 U), respectively. Antiannexin II oligonucleotides significantly inhibited the binding ability of t-PA and plasminogen to annexin II, and obviously reduced the plasminogen activation in vitro. The above findings showed human umbilical vein endothelial cells (HUVECs) treated with sense or missense oligonucleotides indicated no significant change in binding of t-PA and PLG. Treatment of HUVECs with antiannexin II oligonucleotides could significantly reduce the plasminogen activation by 2.4 +/- 0.3 U as compared with sense oligonucleotide group in binding of t-PA and PLG. These results, therefore, suggest that Ann- II can bind plasminogen and participate in the stimulation of t-PA-dependent activation of plasminogen, and that interference with Ann-II mRNA by antisense oligonucleotide may be a new strategy for the therapy of bleeding in patients with hyperfibrinolysis.
