Potential role of human DNA-repair enzymes hMTH1, hOGG1 and hMYHalpha in the hepatocarcinogenesis.
- Author:
Bin CHENG
1
;
Christoph JÜNGST
;
Jusheng LIN
;
Wolfgang H CASELMANN
Author Information
1. Abteilung für Innere Medizin, Tongji Klinikum, Tongji Medizinisches Institut, Huazhong Universität für Wissenschaft und Technik, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Antimutagenic Agents;
Carcinoma, Hepatocellular;
enzymology;
genetics;
DNA Glycosylases;
biosynthesis;
genetics;
DNA Repair;
DNA-Formamidopyrimidine Glycosylase;
biosynthesis;
genetics;
Female;
Humans;
Liver Neoplasms;
enzymology;
genetics;
Male;
Middle Aged;
Oxidants;
pharmacology;
Oxidative Stress
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(3):206-215
- CountryChina
- Language:German
-
Abstract:
To elucidate role of the three enzymes in hepatocarcinogenesis, hMTH1, hOGG1 and hMYH, mRNA expression were examined by using RT/semi-quantitative real-time PCR and 8-O-HdG levels was studied by HPLC/ECD in HCC and non-tumorous liver tissue of 21 patients with hepatocellular carcinoma (HCC). It was found that the 8-OHdG level in non-tumourous liver tissue was significantly higher than in HCC tissue (P = 0.006), and this was correlated with the degree of inflammation. The hMTH1 expression in HCC tissue was significantly higher than in non-tumorous liver tissue (P = 0.014). Inversely, The hMYH alpha expression was significantly increased (P = 0.039) in non-tumorous liver tissue. No difference was seen in hOGG1 expression in non-tumorous liver and HCC tissue. A significant linear correlation between hMTH1 and hOGG1 expression was found both in HCC tissue (r = 0.809, P < 0.001) and in non-tumorous liver tissue (r = 0.883, P < 0.001). Our findings suggested a reactive rather than pathogenic role of the DNA repair enzymes in the hepatocarcinogenesis.
