Qualitative study of three cell culture methods.
- Author:
Aiguo WANG
1
;
Tao XIA
;
Peng RAN
;
Xuemin CHEN
;
Andreas K NUESSLER
Author Information
1. Department of Environmental Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030.
- Publication Type:Journal Article
- MeSH:
Albumins;
secretion;
Animals;
Bioreactors;
Cell Culture Techniques;
methods;
Cell Separation;
Cytochrome P-450 CYP1A1;
metabolism;
Hepatocytes;
cytology;
metabolism;
L-Lactate Dehydrogenase;
metabolism;
Male;
Mitochondria, Liver;
enzymology;
Mitochondrial Proteins;
metabolism;
Rats;
Rats, Sprague-Dawley;
Time Factors
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2002;22(4):288-291
- CountryChina
- Language:English
-
Abstract:
Primary rat hepatocytes were cultured using different in vitro models and the enzyme leakage, albumin secretion, and cytochrome P450 1A (CYP 1A) activity were observed. The results showed that the level of LDH was decreased over time in culture. However, on day 5, LDH showed a significant increase in monolayer culture (MC) while after day 8 no LDH was detectable in sandwich culture (SC). The levels of AST and ALT did not change significantly over the investigated time. The CYP 1A activity was gradually decreased in a time-dependent manner in MC and SC. The decline of CYP 1A was faster in MC than in SC. This effect was partially reversed by using cytochrome P450 (CYP450) inducer such as Omeprazol and 3-methylcholanthrene (3-MC) and the CYP 1A induction was always higher in MC than in SC. In bioreactor basic CYP 1A activity was preserved over 2 weeks and the highest albumin production was observed in bioreactor followed by SC and MC. Taken together, it was indicated each investigated model had its advantages and disadvantages. It was also underlined that various in vitro models may address different questions.
