The inhibitory effects of an antisense u-PAR vector on invasion of highly invasive human prostate carcinoma PC-3M cell subclones.
- Author:
Guoning LIAO
1
;
Qingfen LI
;
Youmei FENG
;
Yaozu DENG
;
Zhuoya LI
;
Feili GONG
;
Ding MA
Author Information
1. Department of Biochemistry and Molecular Biology, Molecular Cancer Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Animals;
Antisense Elements (Genetics);
genetics;
pharmacology;
Cell Division;
drug effects;
Cloning, Molecular;
Humans;
Male;
Matrix Metalloproteinase 9;
genetics;
metabolism;
Mice;
Mice, Inbred BALB C;
Mice, Nude;
Neoplasm Invasiveness;
Prostatic Neoplasms;
metabolism;
pathology;
RNA, Antisense;
Receptors, Cell Surface;
genetics;
metabolism;
Receptors, Urokinase Plasminogen Activator;
Reverse Transcriptase Polymerase Chain Reaction;
Transfection;
Tumor Cells, Cultured
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(2):101-104
- CountryChina
- Language:English
-
Abstract:
To observe the inhibitory effects of an antisense u-PAR vector on invasion of highly invasive PC-3M cell subclones, the effects of the antisense u-PAR on activity of MMP-9 in those highly invasive cell subclones were detected by a quantitative RT-PCR and zymography. The monolayer invasion assay and colony formation assay in soft agar were used. And tumorigenesis rate and invasions by the cell subclones with or without the antisense u-PAR were observed in nude mice. It was found that in vitro growth of highly invasive PC-3M cell subclones transfected with the antisense u-PAR was declined, and the ability of anchorage-independent growth of those cell subclones was found decreased sharply, with the inhibiting rate becoming 79% and 60%, respectively. Although the antisense u-PAR didn't change MMP-9 gene transcription, they could inhibit the activation of MMP-9 of highly invasive PC-3M cell subclones. Moreover, the tumorigenesis rate of the cell subclones with the antisense u-PAR decreased and the growth of a neoplasm also slowed down. The t tests showed the difference between experimental and control groups was statistically significant (P < 0.01). The antisense u-PAR vector could not only inhibit the invasion ability of highly invasive PC-3M cell subclones in vitro but also restrain the growth of those cell subclones in vivo.
