Construction of antisense transforming growth factor beta 1 gene and its effect on the proliferation by expression in osteosarcoma cells.
- Author:
Yong LIU
1
;
Qixin ZHENG
;
Jingyuan DU
;
Shuhua YANG
;
Zengwu SHAO
;
Baojun XIAO
Author Information
1. Department of Orthopaedics, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
- Publication Type:Journal Article
- MeSH:
Antisense Elements (Genetics);
biosynthesis;
genetics;
Bone Neoplasms;
genetics;
metabolism;
pathology;
Cell Division;
Humans;
Osteosarcoma;
genetics;
pathology;
Transcription, Genetic;
Transforming Growth Factor beta;
biosynthesis;
genetics;
Transforming Growth Factor beta1;
Tumor Cells, Cultured
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(2):163-165
- CountryChina
- Language:English
-
Abstract:
To construct the antisense transforming growth factor beta 1 (TGF beta 1) gene and investigate the effect of TGF beta 1 autocrine loop blockage on the proliferation of osteosarcoma cells. TGF beta 1 cDNA was cloned by RT-PCR from human osteosarcoma cells (MG-63) and inserted into pcDNA3 to construct an antisense expression vector, which was dubbed pcDNA3-TGF beta 1(-). MTT was used to detect the proliferation of osteosarcoma cells transfected by antisense TGF beta 1 gene. Our results showed that the proliferation of the transfected osteosarcoma cells was suppressed markedly. It is concluded that TGF beta 1 autocrine loop blockage in osteosarcoma cells could inhibit cell proliferation, which might be helpful for gene therapy of osteosarcoma.
