Detection of embryo sex chromosome by dual color fluorescent in-situ hybridization.
- Author:
Qun LIU
1
;
Guijin ZHU
Author Information
1. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
- Publication Type:Journal Article
- MeSH:
Blastocyst;
Chromosomes, Human, X;
genetics;
Chromosomes, Human, Y;
genetics;
Embryonic Development;
Female;
Humans;
In Situ Hybridization, Fluorescence;
Mosaicism;
genetics;
Pregnancy;
Preimplantation Diagnosis;
methods;
Sex Chromosome Aberrations;
embryology;
Sex Chromosome Disorders;
diagnosis;
Sex Determination Processes
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(2):187-189
- CountryChina
- Language:English
-
Abstract:
In order to evaluate the effects of sex chromosomal mosaicism on the accuracy of single-cell gender diagnosis, sex chromosomes of 21 normal fertilized embryos were detected by dual color fluorescent in-situ hybridization (FISH). The results showed that 4 embryos had sex chromosomal mosaicism (19%) and the remaining 17 showed uniformly XX or XY signals in all blastomeres. In conclusion, identification of sex by dual color FISH analysis of a single cell was accurate and efficient, and sex chromosomal mosaicism would not affect preimplantation gender diagnosis.
