Subtype and sequence analysis of human immunodeficiency virus type-1 strains in Guangdong province.
- Author:
Zhuo-yue WAN
1
;
Hui XING
;
Jie LI
;
Yu WANG
;
Li-mei DIAO
;
Peng-fei MA
;
Peng LIN
;
Shu-lin JIANG
;
Yi-ming SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Acquired Immunodeficiency Syndrome; blood; epidemiology; virology; Adolescent; Adult; Base Sequence; Child; China; epidemiology; DNA, Viral; Female; HIV-1; classification; genetics; Humans; Male; Middle Aged; Protein Isoforms
- From: Chinese Journal of Preventive Medicine 2006;40(5):344-347
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate subtype and genetic analysis of human immunodeficiency virus type-1 (HIV-1).
METHODSDNA sequences were amplified by nested-PCR from uncultured peripheral blood mononuclear cells (PBMC) obtained from 100 HIV-1 patients from Guangdong Province. The C2 to V3 region of the envelope glycoprotein gp120 of HIV-1 was sequenced directly. The analysis of the gene-based phylogenetic tree and variation of amino acid were carried out by using Wisconsin software package or genetics computer group (GCG).
RESULTSDNA fragments were amplified from 75 PBMC samples by using nested polymerase chain reaction (PCR). Sequence analysis showed that there were 3 HIV-1 subtypes or circulating recombinant forms (CRF): CRF01-AE (n = 44), CRF-BC (n = 27) and B' (n = 4).
CONCLUSIONSThree HIV-1 subtypes or circulating recombinant forms: CRF01-AE, CRF-BC and B' might be circulating in Guangdong Province. Findings from this study suggested that several subtypes might exist in Guangdong Province and the epidemic situation of AIDS be serious. It should be a challenge for Guangdong Province in treating patients, preventing and controlling AIDS in the future.
