Association between nucleotide excision repair gene polymorphisms and chromosomal damage in coke-oven workers.
- Author:
Juan CHENG
1
;
Shu-Guang LENG
;
Yu-Fei DAI
;
Zu-Fei PAN
;
Yong NIU
;
Bin LI
;
Yu-Xin ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alcohol Drinking; Coke; Comet Assay; DNA Damage; DNA Repair; genetics; DNA Repair Enzymes; genetics; Dihydroxydihydrobenzopyrenes; urine; Extraction and Processing Industry; Female; Gene Frequency; Genetic Predisposition to Disease; Genotype; Humans; Lymphocytes; Male; Micronucleus Tests; Middle Aged; Occupational Exposure; analysis; Polymorphism, Genetic; Smoking
- From: Chinese Journal of Preventive Medicine 2006;40(6):400-404
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association of polymorphisms of nucleotide excision repair genes and chromosomal damage in peripheral blood lymphocytes among coke-oven workers.
METHODSThe genotypes of ERCC1 C19007T, ERCC2 C22541A, ERCC2 G23591A, ERCC2 A35931C, ERCC4 T30028C, ERCC5 G3507C and ERCC6 A3368G among 140 coke-oven workers and 66 non-coke-oven controls were determined by PCR-PFLP methods. Chromosomal damage was detected by cytokinesis-block micronucleus (CBMN) assay.
RESULTSMultivariate analysis of covariance revealed that in coke-oven workers, the ERCC1 19007 CC genotype exhibited significantly higher CBMN frequency [(1.05 +/- 0.68)%] than did the CT [(0.81 +/- 0.66)%] (P = 0.01) or TT [(0.66 +/- 0.37)%] (P = 0.05) or CT + TT genotypes [(0.75 +/- 0.63)%] (P = 0.004). For the ERCC6 A3368G polymorphism, AA genotype exhibited significantly higher CBMN frequency [(1.00 +/- 0.69)%] than did the AG [(0.67 +/- 0.42)%] (P = 0.05) or AG + GG genotypes [(0.66 +/- 0.41)%] (P = 0.02). Stratification analysis found the significant association between the two polymorphisms, ERCC1 C19007T and ERCC6 A3368G, and the CBMN frequencies were most pronounced in older workers. In addition, for the polymorphism of ERCC2 G23591A, GA carriers had significantly higher CBMN frequencies [(1.40 +/- 0.63)%] than those GG carriers [(0.98 +/- 0.59)%] (P = 0.01) in older workers.
CONCLUSIONSOur results suggested that polymorphisms of ERCC1 C19007T, ERCC6 A3368G and ERCC2 G23591A were associated with the CBMN frequencies in coke-oven workers.
