Restoration of Brain Acid Soluble Protein 1 Inhibits Proliferation and Migration of Thyroid Cancer Cells.

Run-sheng Guo; Yue YU; Jun Chen; Yue-yu Chen; Na Shen; Ming Qiu

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Restoration of Brain Acid Soluble Protein 1 Inhibits Proliferation and Migration of Thyroid Cancer Cells.

Author: Run-Sheng GUO ^{1
,

2} ; Yue YU ³ ; Jun CHEN ⁴ ; Yue-Yu CHEN ⁵ ; Na SHEN ⁶ ; Ming QIU ⁷
Author Information

1. Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 201705
2. Department of General Surgery, Jiading Central Hospital, Shanghai 201822, China.
3. Department of General Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
4. Department of Pathology, Jiading Central Hospital, Shanghai 201822, China.
5. Department of General Surgery, Jiading Central Hospital, Shanghai 201822, China.
6. Department of Otorhinolaryngology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
7. Department of General Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 201705, China.
Publication Type:Journal Article
MeSH: Aged; Animals; Apoptosis; genetics; physiology; Calmodulin-Binding Proteins; genetics; metabolism; Cell Cycle; genetics; physiology; Cell Line, Tumor; Cell Movement; genetics; physiology; Cell Proliferation; genetics; physiology; Cytoskeletal Proteins; genetics; metabolism; Female; Gene Expression Regulation, Neoplastic; genetics; physiology; Humans; Male; Membrane Proteins; genetics; metabolism; Mice; Mice, Nude; Middle Aged; Nerve Tissue Proteins; genetics; metabolism; Repressor Proteins; genetics; metabolism; Thyroid Neoplasms; metabolism; pathology; Xenograft Model Antitumor Assays
From: Chinese Medical Journal 2016;129(12):1439-1446
CountryChina
Language:English
Abstract:
BACKGROUNDBrain acid soluble protein 1 (BASP1) is identified as a novel potential tumor suppressor in several cancers. However, its role in thyroid cancer has not been investigated yet. In the present study, the antitumor activities of BASP1 against the growth and migration of thyroid cancer cells were evaluated.
METHODSBASP1 expression in thyroid cancer tissues and normal tissues were examined by immunohistochemical staining and the association between its expression and prognosis was analyzed. pcDNA-BASP1 carrying full length of BASP1 cDNA was constructed to restore the expression of BASP1 in thyroid cancer cell lines (BHT-101 and KMH-2). The cell proliferation in vitro and in vivo was evaluated by WST-1 assay and xenograft tumor models, respectively. Cell cycle distribution after transfection was analyzed using flow cytometry. Cell apoptosis after transfection was examined by annexin V/propidium iodide assay. The migration was examined using transwell assay.
RESULTSBASP1 expression was abundant in normal tissues while it is significantly decreased in cancer tissues (P = 0.000). pcDNA-BASP1 restored the expression of BASP1 and significantly inhibited the growth of BHT-101 and KMH-2 cells as well as xenograft tumors in nude mice (P = 0.000). pcDNA-BASP1 induced G1 arrest and apoptosis in BHT-101 and KMH-2 cells. In addition, pcDNA-BASP1 significantly inhibited the cell migration.
CONCLUSIONSDownregulation of BASP1 expression may play a role in the tumorigenesis of thyroid cancer. Restoration of BASP1 expression exerted extensive antitumor activities against growth and migration of thyroid cancer cells, which suggested that BASP1 gene might act as a potential therapeutic agent for the treatment of thyroid cancer.