Inhibitory effects of recombinant adenovirus carrying human endostatin gene on the growth of human pancreatic carcinoma xenograft in nude mice.
- Author:
Luo-sheng ZHANG
1
;
Ben-fu HE
;
Xing-wang GAO
;
Li-xia WEI
;
Na MIN
;
Xian-rong LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; metabolism; Angiogenesis Inhibitors; metabolism; pharmacology; Animals; Endostatins; biosynthesis; genetics; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Neovascularization, Pathologic; genetics; Pancreatic Neoplasms; blood supply; pathology; therapy; Recombinant Proteins; biosynthesis; genetics; pharmacology
- From: Journal of Southern Medical University 2010;30(4):878-880
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the inhibitory effect of recombinant adenovirus carrying human endostatin gene (Ad-endo) on the growth of human pancreatic carcinoma xenograft in nude mice.
METHODSThe expression of endostatin in human pancreatic carcinoma Capan-2 cells was examined by RT-PCR after infection with Ad-endo. The supernatants of Capan-2 cells were collected after 48 h of infection with Ad-endo as the conditioned medium for human umbilical vein endothelial cells (HUVECs), whose proliferation in vitro was assayed. Capan-2 cell xenografts were established to determine the antitumoral effects of Ad-endo in vivo. The intratumoral microvessel density (MVD) was evaluated using CD31 staining.
RESULTSThe expression of endostatin gene was detected by PT-PCR in infected Capan-2 cells. The conditioned medium from Ad-endo-infected cells significantly inhibited HUVEC proliferation (P<0.05). Ad-endo significantly suppressed the growth of Capan-2 tumor xenografts in nude mice (P<0.05), and the MVD decreased significantly in the treated tumor (P<0.05) as compared with that in the control group.
CONCLUSIONAdenovirus carrying human endostatin gene produces inhibitory effects on the growth of human pancreatic carcinoma tumors in nude mice.
