Effect of polyethylene oxide on red blood cell velocity in rat cremaster microcirculation.
- Author:
Rong-sheng DU
1
;
Dao-gang ZHA
;
Bing-jie ZHOU
;
Feng HU
;
Li-jing JI
;
Jue-fei WU
;
Jian-ping BIN
;
Yi-li LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Flow Velocity; drug effects; Male; Microcirculation; drug effects; physiology; Muscle, Smooth; blood supply; Polyethylene Glycols; pharmacology; Rats; Rats, Wistar; Testis
- From: Journal of Southern Medical University 2010;30(5):960-962
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the drag-reducing effect of polyethylene oxide (PEO) on the velocity of red blood cells in rat cremaster microcirculation.
METHODSBlood samples were collected from 6 Wistar male rats (100-110 g) via the post-orbital venous plexus. The red blood cells were separated by centrifugation and labeled by fluorescinisothiocyate (FITC). After successful establishment of cremaster model, the labeled red blood cells were injected into the jugular vein, and the microcirculation was observed and recorded under fluorescence microscope. The hemodynamic parameters and microcirculation video was recorded every 4 min since 4 min before PEO or normal saline injection. Both PEO (10 ppm) and normal saline was injected into the same rat in random sequence at a constant rate of 3.5 ml/h for 20 min followed by observation for another 20 min. The velocity of the labeled-red blood cells was determined by IPP 6.0 software.
RESULTSCompared with normal saline, PEO significantly increased the velocity of the red blood cells in the rat cremaster microcirculation (498.7-/+182.89 microm/s vs 773.54-/+308.27 microm/s, P=0.012). No significant changes in the heart rate and arterial blood pressure were observed during the experiment (P=0.836, P=0.420).
CONCLUSIONPEO at an extremely low concentration can significantly increase the velocity of the red blood cells in rat cremaster microcirculation and produces no significant impact on heart rate and arterial blood pressure.