Dimethylsulfoxide-induced expression of thymic stromal lymphopoietin in human bronchial epithelial cells.
- Author:
Dan ZHANG
1
;
Shao-xi CAI
;
Hai-jin ZHAO
;
Lai-yu LIU
Author Information
- Publication Type:Journal Article
- MeSH: Bronchi; cytology; metabolism; Cell Proliferation; drug effects; Cells, Cultured; Cytokines; genetics; metabolism; Dimethyl Sulfoxide; pharmacology; Epithelial Cells; metabolism; Humans; RNA, Messenger; genetics; metabolism
- From: Journal of Southern Medical University 2010;30(5):963-965
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of dimethylsulfoxide on the expression of thymic stromal lymphopoietin (TSLP) in human bronchial epithelial cell (HBE).
METHODS16HBE cells were incubated in the presence of dimethylsulfoxide at different concentrations, and the cell proliferation changes were observed. The expressions of TSLP mRNA and protein in the cells were detected by real-time quantitative PCR and ELISA, respectively.
RESULTSDimethylsulfoxide induced significantly increased TSLP mRNA expression in HBE cells (P<0.01) in a concentration-dependent manner. The level of TSLP protein in the supernatant was also increased after dimethylsulfoxide treatment, but high concentration of dimethylsulfoxide resulted in e inhibited cell proliferation.
CONCLUSIONDimethylsulfoxide may affect the immunomodulatory function of HBE cells.
